Enterokinase is a heterodimeric serine protease present in the mammalian duodenum. The molecular mass is 150ku, and it consists of one 115ku heavy chain and one 35ku light chain, and it specifically hydrolyzes protein substrates at pH 4.5-9.5 and temperature 4-45°C.
Recombinant enterokinase is a highly purified recombinant bovine enterokinase light chain fragment.
Recombinant enterokinase is used in the development of genetic engineering products, especially in bioengineering pharmaceutical industry and genetic engineering, biochemistry, molecular biology and other research. Used to remove fusion proteins located at the N-terminus of proteins to remove unwanted fusion tags.
1) To obtain ideal enzyme digestion results, please dialyze the sample into 25mM Tris-HCl 8.0 buffer, and then perform enzyme digestion.2) If it is not convenient for dialysis, the sample can be diluted, the imidazole content is below 100mM, the NaCl concentration is below 50mM, and the glycerol concentration is below 5% for enzyme digestion. .3) If the sample solution contains one or more of the above components and it is inconvenient to remove, at this time, appropriately increasing the amount of enzyme or prolonging the digestion time can also achieve a better digestion effect.
2) If it is not convenient for dialysis, the sample can be diluted, the imidazole content is below 100mM, the NaCl concentration is below 50mM, and the glycerol concentration is below 5% for enzyme digestion. .3) If the sample solution contains one or more of the above components and it is inconvenient to remove, at this time, appropriately increasing the amount of enzyme or prolonging the digestion time can also achieve a better digestion effect.
3) If the sample solution contains one or more of the above components and it is inconvenient to remove, at this time, appropriately increasing the amount of enzyme or prolonging the digestion time can also achieve a better digestion effect.
No animal origin: recombinant production, no exogenous virus contamination, no animal origin raw materials are used in the production process.
Stable quality: Mass production can ensure stable and continuous batch production; there is no difference between product batches, and the quality is stable.
High purity: no other proteases, no non-specific cleavage.
High output: ton-level tank production, suitable for industrial customers.
Compliance with regulatory requirements: The production equipment and production environment comply with relevant regulatory requirements, and the production process fully follows the NSF ISO 9001:2015 quality system and complies with GMP guidelines.
1) The molecular weight is small. MW is about 26KDA2) Electrophoretic purity. SDS-PAGE single major band3) The remaining amount of DNA and HCP is small.4) High activity. Activity not less than10U/ul.
Kekai’s recombinant enterokinase has been used by many pharmaceutical companies in china and overseas countries, and achieved good results.