1. Product Introduction
Recombinant Carboxypeptidase B is exclusively used for the hydrolysis of protein C-terminal basic amino acids (lysine, arginine, histidine); also known as peptidyl-L-lysine (L -Arginine) hydrolase; sperminase. The molecular weight is 35kD, the isoelectric point is 6.0, and the optimum pH is 7-9. The activity is competitively inhibited by arginine and lysine, and metal ion chelating agents such as EDTA inhibit enzyme activity.
2. Product advantages
No animal origin: recombinant production, no foreign virus contamination, such as swine flu virus, porcine parvovirus, etc.
High purity: no other miscellaneous enzyme activity: no other side reactions in the digestion reaction, high yield
High specific activity: no less than 120 u/mg pro.; no less than 170 u/mg pro.
Electrophoresis purity: SDS-PAGE electrophoresis identification, 95%
Stable: freeze-dried powder, easy to store and transport
Figure 1. Diagram of the production process of different levels of non-animal origin of biological products
Recombinant carboxypeptidase B is a product of non-animal origin AOF3 level
3. Product use
Production of recombinant insulin and its analogues
Determination of protein C-terminal amino acid
Production of other recombinant peptides
Enzymatic synthesis of some special compounds
4. Product features
Source: Recombinant Escherichia coli
Product properties: white or almost white freeze-dried powder
Electrophoresis identification and Coomassie brilliant blue staining
12%SDS-PAGE: molecular weight 35kD, single main band
Specific activity: not less than 120 u/mg pro.; not less than 170 u/mg pro.
Other enzyme content: no chymotrypsin, carboxypeptidase A, etc., trypsin is not detected. Trypsin content is less than 10ppm
Does not contain any protease inhibitors: No protease inhibitors such as PMSF, EDTA, etc.
Figure 2. SDS-PAGE identification and purity analysis of recombinant carboxypeptidase B freeze-dried powder
Our product consultants have received our technical training on organic and conventional ingredients, and they are full of ideas on how to make your products better. Coupled with our extraordinary customer service, you will have a unique supplier of raw materials. Contact us and let us change your view of raw material suppliers. You can choose to send us your requirements via the form. Or send an email to firstname.lastname@example.org