Recombinant Carboxypeptidase B

Recombinant Carboxypeptidase B

Genetic engineering production, no animal origin, no virus pollution, no miscellaneous enzyme pollution

CAS:9025-24-5
EC:3.4.17.2
Description
Additional information

1. Product Introduction

Recombinant Carboxypeptidase B  is exclusively used for the hydrolysis of protein C-terminal basic amino acids (lysine, arginine, histidine); also known as peptidyl-L-lysine (L -Arginine) hydrolase; sperminase. The molecular weight is 35kD, the isoelectric point is 6.0, and the optimum pH is 7-9. The activity is competitively inhibited by arginine and lysine, and metal ion chelating agents such as EDTA inhibit enzyme activity.

2. Product advantages

No animal origin: recombinant production, no foreign virus contamination, such as swine flu virus, porcine parvovirus, etc.

High purity: no other miscellaneous enzyme activity: no other side reactions in the digestion reaction, high yield

High specific activity: no less than 120 u/mg pro.; no less than 170 u/mg pro.

Electrophoresis purity: SDS-PAGE electrophoresis identification, 95%

Stable: freeze-dried powder, easy to store and transport

Figure 1. Diagram of the production process of different levels of non-animal origin of biological products

Recombinant carboxypeptidase B is a product of non-animal origin AOF3 level

3. Product use

Production of recombinant insulin and its analogues

Determination of protein C-terminal amino acid

Production of other recombinant peptides

Enzymatic synthesis of some special compounds

4. Product features

Source: Recombinant Escherichia coli

Purification: HPLC

Product properties: white or almost white freeze-dried powder

Electrophoresis identification and Coomassie brilliant blue staining

12%SDS-PAGE: molecular weight 35kD, single main band

Specific activity: not less than 120 u/mg pro.; not less than 170 u/mg pro.

Other enzyme content: no chymotrypsin, carboxypeptidase A, etc., trypsin is not detected. Trypsin content is less than 10ppm

Does not contain any protease inhibitors: No protease inhibitors such as PMSF, EDTA, etc.

Figure 2. SDS-PAGE identification and purity analysis of recombinant carboxypeptidase B freeze-dried powder

Product No
KK-T004
Product name
Recombinant carboxypeptidase B
Activity
≥170u/mg pro.
Packing
10mg,1g, tube or bottle or bag etc
Activity unit
25°C, pH 7.65, the amount of enzyme that catalyzes the hydrolysis of 1 μmol hippuryl-L-arginine per minute is defined as an enzyme activity unit.
Feedback
                   

Our product consultants have received our technical training on organic and conventional ingredients, and they are full of ideas on how to make your products better. Coupled with our extraordinary customer service, you will have a unique supplier of raw materials. Contact us and let us change your view of raw material suppliers. You can choose to send us your requirements via the form. Or send an email to sales@koka-bio.com