Name: Sticky Recombinant Streptavidin (S-rSA)
Specification: 1 mg, 10 mg, 100 mg, 500 mg
Product form: lyophilized powder, the solution before lyophilization is 5mM PB (4M Na2HPO4, 1mM NaH2PO4, pH7.4) solution
Molecular weight: subunit monomer 23.5kDa, tetramer 94kDa
Isoelectric point: 6.04
Theoretical activity: 18.1 U/mg protein (S-rSA: Biotin=1:4 (mol: mol))
Activity: ≥ 15 U/mg protein (determined by modified Green method)
Purity: ≥ 95% (SDS-PAGE detection)
Concentration determination: 280nm absorption method, molar absorption coefficient 1.68 × 105 (mol/L) – 1 • cm-1, that is, c (g/L)=A280nm/2.00
Endotoxin: ≤ 5EU/mg
Source: Escherichia coli
Storage condition: – 20 ℃
Validity: 2 years
Streptavidin (SA) is a homotetramer protein secreted by Streptomyces avidinii during its growth. Like avidin, one mole of streptavidin can bind four moles of biotin, which has high affinity with biotin. Because SA does not contain sugar groups, it has a lower non-specific binding level than avidin in detection and application, and has been widely used in enzyme-linked immunosorbent assay, immunohistochemistry, time-resolved immunofluorescence (TRFIA), quantitative PCR, single-stranded DNA preparation, biological component purification, monoclonal antibody preparation and other biotechnology fields.
However, the adsorbability of streptavidin is poor, and it is difficult to adsorb onto NC membrane or embed it in practical applications. Sticky recombinant streptavidin (S-rSA) is mainly used to solve this problem. It not only has the core sequence related to activity, but also has better adsorbability, stability, solubility and other aspects than natural SA, especially on NC membrane.
Scope of application
In many applications, such as immunocytochemistry, ELISA and immunoblotting, streptavidin alkaline phosphatase can be used to detect biotinylated molecules, such as antibodies.
For most applications, 1:150 working dilution in TBS is sufficient. At this dilution, about 3000 tests (100ul per slide) can be carried out for each package. It is recommended to use TBS instead of PBS in all diluents and washings to maintain maximum enzyme activity.
Our product consultants have received our technical training on organic and conventional ingredients, and they are full of ideas on how to make your products better. Coupled with our extraordinary customer service, you will have a unique supplier of raw materials. Contact us and let us change your view of raw material suppliers. You can choose to send us your requirements via the form. Or send an email to firstname.lastname@example.org