Proline hydroxylase (polypeptide or Prolyl-4-hydroxylase) (EC 184.108.40.206) is an important family of dioxygenases α- Ketoglutaric acid and O2 as co-substrates, depending on Fe
As a cofactor, 2+catalyzes specifically to form trans-4-hydroxy-L-hydroxyproline (L-Hyp). The proline hydroxylase produced by our company is expressed in Escherichia coli and purified by affinity chromatography. Its molecular weight is 29.7 kDa, and it contains 6 His-tag. The optimal enzyme digestion PH is 6.0~8.0, 37 ° C. The activity was inhibited by bivalent cations, proline analogues, pyridine 2,4 (5) – dicarboxylate, 3,4-dihydroxybenzoate, lactam antibiotics, anthracycline substances.
Industrial production: catalyze L-proline to L-hydroxyproline for use in the fields of food, medicine and beauty. Medical cosmetology: Collagen, as an extracellular matrix protein, can account for three quarters of dry weight, especially in skin cell tissue. Proline in mature collagen is catalysed by proline hydroxylase into hydroxyproline in different degrees, and then forms a stable triple helix fold structure, which makes collagen have good tensile strength. The recombinant proline hydroxylase of this product can be used as a biological active ingredient to reduce skin fine lines and restore skin elasticity and smoothness.
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