Recombinant Luciferase is the cloning and recombinant expression of luciferase gene from the North American firefly (Photinus pyralis), which provides credibility and reliability for experimental research or the use of biological fluorescence reagent preparation kit to detect ATP or luciferin substrate. This recombinant enzyme can overcome the influence of seasonal and local regional changes in the purification of luciferase from natural resources.
Luciferase can catalyze the oxidation of luciferin to oxyluciferin. In the process of luciferin oxidation, it will emit biofluorescence. The biofluorescence released in the process of luciferin oxidation can be measured by fluorometer equipment. It is often used in the research of promoter transcription activity regulation and miRNA target gene verification.
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