Recombinant human basic fibroblast growth factor

Name: Recombinant Human Fibroblast Growth Factor-basic (rh-bFGF)

Specification: 10 µ g, 100 µ g, 1 mg, specially customized

Product form: freeze-dried powder, the solution before freeze-dried is 5 mM PB (pH 7.4)

Molecular weight: 17.2 kDa

Isoelectric point: 9.58

A280nm(1 mg/mL)：0.945

Source: Escherichia coli

Purity: ≥ 95% (SDS-PAGE detection)

Endotoxin:<5.0 EU/mg protein

Storage condition: – 20 ℃

Validity: 2 years

Activity: EC50=0.25~1 ng/ml, corresponding activity is 1 * 106~4 * 106 U/mg protein (dose dependent proliferation test of mouse BALB/c 3T3 cells)

Related introduction:

Fibroblast growth factor-basic (bFGF), also known as FGF-2, can combine with heparin and is a member of the FGF superfamily. It plays an important role in embryo formation, tissue regeneration, wound healing, central nervous system development, angiogenesis and tumor development. BFGF is an important mitogenic factor, which has strong mitogenic and proliferative activity on mesoderm-derived cells such as BALB/c 3T3 fibroblasts, capillary and endocardial cells, myoblasts, vascular smooth muscle cells, mesothelial cells, glial and astrocytes, and adrenocortical cells.

Recombinant human bFGF (rh-bFGF) expressed in Escherichia coli is a non-glycosylated single-peptide protein with 155 amino acids and a molecular weight of 17.2 kDa.