Recombinant insulin-like growth factor

-Product Introduction

Insulin-like growth factor-1 (IGF-1), also known as SomatamedinC, belongs to the insulin-like growth factor family and has structural homology with proinsulin. IGF-1 is a kind of polypeptide that promotes mitosis. It stimulates the proliferation and survival of muscle, bone, cartilage and other cells in vitro. IGF-1 can combine with IGF-1R, IGF-2R and insulin receptor, awaken multiple signal pathways, and participate in cell proliferation, differentiation, survival, growth, apoptosis and regeneration. Recombinant human insulin-like growth factor (IGF-1), composed of 70 amino acids, is a bioactive protein for cell culture. This strain was expressed by recombinant yeast, purified by multi-step separation, and then 0.22 μ M The lyophilized powder is made by freeze-drying after filtration and bacteria removal.

-Product advantages

No animal origin: no animal virus, no pathogenic substances, no foreign factor pollution, high safety and security.

Quality stability: This product is a lyophilized powder containing biological active protective agent. It can be produced in batches with small difference between batches.

The production scale is more than 1000L.

Compliance: The production equipment and production environment comply with the requirements of relevant laws and regulations, and comply with the guiding principles of GMP.

-Product characteristics

Speciesman

Source Pichia pastoris

White or almost white lyophilized powder

Purity ≥ 95% (SDS-PAGE)

Specific activity ≥ 2.2 × 105 IU/mg pro.

Molecular weight 7.7 KD ± 0.8kDa

Endotoxin<0.2 EU/µ g

Isoelectric point 8.4 – 8.6

The dissolution point has good solubility in water, buffer solution and culture medium

Biological activity: The biological activity of IGF-1 was determined by its stimulating effect on human breast cancer cell line MCF-7.

-Product use

Widely used in cell culture, it can enhance cell proliferation, cell growth and cell differentiation.

-Usage

It is recommended that the powder be precipitated at the bottom of the tube by centrifugation for a short time before capping, and then dissolved with sterilized deionized water to prepare 100 μ G/ml storage solution can be stored stably for one week at 2 ℃~8 ℃. Dilute to a lower concentration (not less than 10 μ G/ml), 0.1% tissue culture grade BSA or HSA needs to be added. If the storage time needs to be extended, it is recommended to freeze at – 80 ℃ or – 20 ℃, and it is not recommended to freeze and thaw repeatedly.

The best concentration of this product should be determined by experiment according to each specific application.

-Transport and storage stability

Transportation stability: ice bag transportation, stable activity.

Storage stability: stable to 24 months at – 15 ℃ and below, and to 4 weeks at 2~8 ℃. Compounded as 100μ G/ml storage solution can be stored stably for one week at 2~8 ℃.

-Notes and Disclaimer

This product should not be stored for a long time in high temperature environment.

Avoid contamination during the operation of this product.

This product is for research use only, and shall not be used for diagnosis or treatment of animals or humans.