Recombinant protein A (referred to as recombinant Staphylococcus aureus protein A or rSPA) is expressed in recombinant E. coli and purified by HPLC.
Recombinant Staphylococcus aureus protein A was expressed in recombinant E. coli and purified by HPLC.
Form： Lyophilized powder
Ingredients： The recombinant protein A aqueous solution is directly freeze-dried without any other additives.
Purity： (SDS-PAGE detection) >95%
Nucleic acid (UV detection)： DNA, RNA cannot be detected
Triton content： No Triton pollution
Store： at 2℃~8℃
pH stable range： 1-13
1）. Purification by non-antibody column, without any contamination of other antibodies such as IgG in the product.
2）. SPA-25 is a product independently developed by the company. It has a wide range of pH tolerance, can tolerate 0.5 M NaOH and 0.5 M HCl treatment, does not degrade, and the antibody binding ability remains unchanged.
3）. The product has a connecting group specially designed for antibody purification column, which can be directly used for the preparation of protein A column.
4）. The company’s recombinant protein A products have the advantages of large-scale production, stable quality and high purity.
Recombinant protein A can be coupled to a solid separation medium (such as agarose) for the purification of monoclonal antibodies or polyclonal antibodies. Recombinant protein A can be coupled with a variety of reporter molecules (fluorescent dyes, enzyme labels, biotin, colloidal gold, radioactive labels, etc.) without affecting its binding activity to antibodies. These coupled protein A derivatives can be used in Western-blot, ELISA, immunohistochemistry and other antibody detection.
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