Recombinant Protein A

1. Alias

Recombinant  protein A (referred to as recombinant Staphylococcus aureus protein A or rSPA) is expressed in recombinant E. coli and purified by HPLC.

2. Source

Recombinant Staphylococcus aureus protein A was expressed in recombinant E. coli and purified by HPLC.

3. Product features and advantages

Form： Lyophilized powder

Ingredients： The recombinant protein A aqueous solution is directly freeze-dried without any other additives.

Purity： (SDS-PAGE detection) >95%

Nucleic acid (UV detection)： DNA, RNA cannot be detected

Triton content： No Triton pollution

Store： at 2℃~8℃

pH stable range： 1-13

1）. Purification by non-antibody column, without any contamination of other antibodies such as IgG in the product.

2）. SPA-25 is a product independently developed by the company. It has a wide range of pH tolerance, can tolerate 0.5 M NaOH and 0.5 M HCl treatment, does not degrade, and the antibody binding ability remains unchanged.

3）. The product has a connecting group specially designed for antibody purification column, which can be directly used for the preparation of protein A column.

4）. The company’s recombinant protein A products have the advantages of large-scale production, stable quality and high purity.

4. Product use

Recombinant protein A can be coupled to a solid separation medium (such as agarose) for the purification of monoclonal antibodies or polyclonal antibodies. Recombinant protein A can be coupled with a variety of reporter molecules (fluorescent dyes, enzyme labels, biotin, colloidal gold, radioactive labels, etc.) without affecting its binding activity to antibodies. These coupled protein A derivatives can be used in Western-blot, ELISA, immunohistochemistry and other antibody detection.