Recombinant Kex-2 protease (e.c.22.214.171.124) belongs to the subtilisin family. It is a precursor processing enzyme encoded by yeast itself, a Ca2 + – dependent serine protease. It can specifically recognize and cleave the carboxy terminal peptide bond of double basic amino acids such as Lys Arg, Arg Arg and pro arg. Unlike trypsin, this enzyme can not recognize and cleave the carboxy terminal peptide bond of single basic amino acid, namely Arg or Lys. Kex2 protease activity was not inhibited by conventional serine protease inhibitors such as aprotinin, PMSF and TPCK. The action pH is pH9.0, and the stable storage pH is ph5.0-6.0.
This product is obtained by the secretory expression technology of recombinant Pichia pastoris and is a recombinant Kex2 protease with high purity and activity.
L no animal origin: no animal virus, no pathogenic substance, no foreign factor pollution, high safety.
L high purity and high specific activity.
L stable quality and batch production, which can ensure stable and continuous batch production; There is no difference between product batches, and the production scale is more than 1000L.
L compliance: the production equipment and production environment meet the requirements of relevant regulations and GMP guidelines.
L complete quality documents: according to customer needs, relevant regulatory support documents can be provided.
Properties: white or off white powder
Molecular weight: 70.0 ± 7.0 kDa
Enzyme activity ≥ 10u / Mg Pro
Foreign DNA residue ≤ 100ng / Mg pro
Bacterial protein residue ≤ 100ng / Mg pro
Unit of activity: the catalytic substrate BOC qrr PNA was measured every minute in 3ml reaction at 25oC, 50mm Tris HCl, 2mm CaCl2, ph8.0
Release 1 μ Mol of 4-nitroaniline was defined as one unit of enzyme activity.
L recommended reaction buffer: pH 7.0-9.0, 50mm Tris HCl, 2mm Ca2 + or HEPES, 5mm Ca2 +. If it is not used immediately after dissolution, it is recommended to use 20mm (pH 5.2) NaAc HAC and 2mm Ca2 + buffer to dissolve the lyophilized powder. The final concentration of the enzyme after dissolution is about 1-10mg / ml. it can be stored under – 20 ℃ after sub packaging as required.
L reaction, using pH 7.0-9.0, 50mm Tris HCl, 2mm Ca2 + or HEPES, 5mm Ca2 + as reaction diluent.
Note: the reaction of enzyme is pH9.0, and the stable pH is 5.0-6.0.
L transportation stability: ice bag thermal insulation transportation, stable activity.
L storage stability: freeze dried powder is stable within 24 months after sealed storage at – 15 ℃. Dissolve it in storage buffer (20mm NaAc HAC, 2mm Ca2 +, ph5.2), and store it at – 15oC after dissolution. It will be stable for 6 months.
L please refer to the relevant literature for the specific working concentration, or explore and optimize it through experiments according to the experimental purpose.
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